Human CD32b / FCGR2B Protein (ECD, His Tag) 10259-H08C from Sino Biological, Inc.

Product Specs
ItemHuman CD32b / FCGR2B Protein (ECD, His Tag)
CompanySino Biological, Inc.
Price Supplier Page View Company Product Page
Catalog Number10259-H08C
Conjugate/TagC-His
Purity> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
TypeRecombinant protein
Molecular WeightThe secreted recombinant human CD32b consists of 183 amino acids and predicts a molecular mass of 20.8 KDa. The apparent molecular mass of the protein is approximately 22-29 KDa in SDS-PAGE under reducing conditions due to different glycosylation
SpeciesHuman
Molecule NameFCGR2B
Source/Expression SystemCHO Cells
Activity1.Immobilized Human FcγRIIB/C / CD32b/c recombinant protein (Cat. 10259-H08C) can bind biotinylated human IgG1, The EC50 is 60-180 ng/mL. 2.Loaded Human FcγRIIB/C / CD32b/c recombinant protein (Cat. 10259-H08C) on His1K Biosensor, can bind Rituximab (IgG1) with an affinity constant of 1.5 µM as determined in a BLI assay (Sartorius Octet Red384). 3.Captured IgG1 on proteinA Chip can bind Human FcγRIIB/C / CD32b/c recombinant protein (Cat. 10259-H08C) with an affinity constant of 2.9 µM as determined in an SPR assay (Biacore T200). 4.Captured Human CD32b/c recombinant protein（10259-H08C）on Anti-His Chip can bind IgG1 Fc Protein, His (Cat. 10702-HNAC) with an affinity constant of 37.5 µM as determined in an SPR assay (Biacore T200)
FormatLyophilized from sterile PBS, pH 7.4
NCBI Full Gene NameFc gamma receptor IIb
NCBI Gene AliasesCD32, CD32B, FCG2, FCGR2, FCGR2C, FcGRIIB, FcRII-c, FcgammaRIIb, IGFR2